Isolation and Extraction of Secondary Metabolites of Marine Actinomadura Species and its use Against Oral Pathogens

Abstract

Abstract:

Introduction:Due to the various opportunities as novel physiologically active bio compounds, marine microorganisms have drawn much attention lately. They can offer a variety of uses in many industries like pharmaceuticals, food and detergents. It is now of growing interest in the dental and medical spectrum too. Marine bacteria can be used to combat the growing trend of antibiotic resistance. In this study the isolation and extraction of secondary metabolites of marine Actinomadura species and its use against oral pathogens. Materials and methods: Samples were collected followed by the identification and spore chain formation. The secondary metabolites were isolated and tested at concentrations of 100, 200, 300, 400, 500µg/ml of Streptococcus mutans, Klebsiella pneumonia, Staphylococcus aureus and P. aeroginosa with tetracycline as the control. Results: The extrcation of secondary metabolites and their antimicrobial potential was observed from the different oral pathogens and the zone of inhibition. Conclusion: Isolated samples of Actinomadura species shows effective results at various concentrations.