Green synthesis of Nanocomposite using silver nanoparticles, Sodium Alginate and Papaya leaf extract and its anticariogenic activity.

Abstract

Abstract

Aim:

The current study is concerned with the production of AgNPs and silver sodium alginate nanocomposite employing Carica papaya extract as a stabilizing and reducing agent.Biosynthesized silver nanocomposite antibacterial effectiveness was investigated against oral pathogens such as Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis, and Candida albicans.

Materials and Methods:

In 90 mL of deionized water, 1 mM of precursor silver nitrate (AgNO3) was added. 10 mL of filtered Carica papaya plant extract was added. For 48 hours, the mixture was stirred at 600-700 rpm using a magnetic stirrer. The synthesis of AgNPs was studied using a UV-Vis-double beam spectrophotometer at regular time intervals.The synthesised silver nanocomposite was centrifuged at 10,000 rpm for 10 minutes. The nanocomposite pellet was suspended in deionized water before being centrifuged and lyophilized. Antimicrobial activity against  S. aureus, S. mutans, E. faecalis, and Candida albicans was checked.

Results:

The results show that the antibacterial activity of biosynthesized AgNPs, sodium alginate nanoparticles, and AgNP-based sodium alginate nanocomposite increases in a dose-dependent manner. The high inhibition zone was found in the gram-positive bacteria Staphylococcus aureus as well as gram-negative organism E. faecalis . In  the opportunistic pathogenic yeast Candida albicans, the inhibitory zone was shown to be the smallest.

CONCLUSION:

Using Carica papaya plant extract, AgNPs and AgNP-based sodium alginate nanocomposite were synthesized, and the obtained AgNPs and nanocomposite showed outstanding stability.