Serotyping, Antibiotic Resistance and Molecular Identification of Salmonella Strains Isolated from Poultry Meat in Rabat, Morocco.

Abstract

Introduction

Animals serve as a primary reservoir of potential human pathogens, such as Salmonella spp., which is a central concern for biosecurity and food safety.

Methods

The serotyping test was performed after species identification from a pure and fresh culture of Salmonella isolated on Chromagar-Salmonella [14].

Results

The results of the serotyping test showed the presence of the following serovars: Salmonella Nitra, Salmonella Typhimurium, Salmonella Schwarzengrund, Salmonella Livingstone, Salmonella Enteritidis, Salmonella Bovis Morbificans,

Salmonella Aba, Salmonella Cocody, Salmonella Kentucky, and Salmonella Rechevot.The study of antibiotic resistance in the Salmonella strains isolated in our study showed that 9/18 (50%) of the isolated Salmonella were resistant to Ceftazidime (CAZ 30), 6/18 (30%) to Sulfamethoxazole-Trimethoprim (SXT25), and that all strains were remarkably sensitive to Cefotaxime (CTX30). These tests were followed by sequencing of the 16S rRNA gene of two suspected Salmonella Typhimurium strains: S-15 and S-36. This study was designed for the molecular characterization and determination of the phylogenetic relatedness of Salmonella isolates. Compared with the sequence of strain S-15, the sequences of the reference Salmonella strains NR074910.1 is characterized by the highest 16S rRNA similarity values (99.52%). Compared with the sequence of strain S-36, the sequence of the reference Salmonella strain NR074910.1 is characterized by the highest 16S rRNA similarity value (99.2%).