Pharmacological Evaluation of Amarathus blitum L Aqueous Extract on Adenine Induced CRF Model of Anemia and Muscle Coordination in Rats

Abstract

Introduction: Amaranthus blitum L, (family: Amaranthaceae), is commonly known as spiny amaranth. It is the traditionally used plant as antipyretic, appetizer, diuretic, febrifuge, haematinic and laxative. This study investigated the impact of Amaranthus blitum L aqueous extract (ABLAE) on adenine induced Chronic Renal Failure (CRF) model of anemia to assess the extract’s potential therapeutic benefits.

Objectives: Primary objective of this research is to evaluate impact of Amaranthus blitum L aqueous extract (ABLAE) on chronic renal failure induced anemia, using an adenine induced anemia model. Additionally, to explore the effect of extract on muscular coordination.

Methods: Rats exposed to adenine were treated with Amaranthus blitum L aqueous extract, at doses 200mg/kg, 400mg/kg and the hematological parameters along with biochemical parameters were evaluated in adenine induced CRF model of anemia. Additionally effect of Amaranthus blitum L aqueous extract on muscle coordination were determined by Rota rod test and grip strength measurement.

Results: Results indicate that Amaranthus blitum L aqueous extract (ABLAE), dose dependently increases hematological parameters like RBC count, hemoglobin level and hematocrit percentage along with erythropoietin and transferrin level in adenine induced CRF model of anemia. ABLAE dose dependently increases duration of time that rats spent on the rotating rod in Rota rod test and enhances grip strength, which indicates muscle strengthening effect.

Conclusions: Amaranthus blitum L aqueous extract at doses 200mg/kg, 400mg/kg demonstrates promising anti-anemic potential in adenine-induced CRF model of anemia in rats and improves muscle strength. Further research and development as a novel treatment for chronic kidney diseases induced anemia and muscle coordination at molecular level is required.