Quantitative and Qualitative Estimation of Gallic Acid in Triphala by HPTLC

Abstract

The present study focuses on the quantitative and qualitative estimation of gallic acid in Triphala using High-Performance Thin Layer Chromatography (HPTLC). Standardized HPTLC methods were carefully developed and validated for the simultaneous identification and estimation of these phytochemical markers. Methanolic extracts of the samples were prepared, analysed, and compared against authentic standard compounds to ensure accuracy and reproducibility. The results revealed significant variations in the concentration of gallic acid, polyherbal formulation indicating the synergistic and balanced contribution of each fruit to the overall efficacy of Triphla.

This investigation clearly validates the application of HPTLC as a reliable, rapid, and cost-effective analytical tool for the quality control, authentication, and standardization of Ayurvedic formulations. In addition, the study highlights the critical role of marker-based standardization in addressing the challenges of variability in herbal medicines caused by environmental, seasonal, and post-harvest factors. By establishing gallic acid as chemical markers, the present work provides a scientific benchmark that can be adopted for regulatory purposes and industrial-scale production. Furthermore, the findings strengthen the evidence supporting the antioxidant potential, rejuvenating activity, and therapeutic relevance of Triphla, thereby bridging the gap between traditional wisdom and modern pharmacological validation. Ultimately, this study promotes the safe, effective, and globally acceptable use of Triphla as a standardized natural remedy in complementary and alternative medicine systems.