Silver Nanoparticles: Biosynthesis Using Pseudomonas Aeruginosa ATCC 27853, Its Characterization and Study of Its Antibacterial Activity Against ESBL Producers

Abstract

Introduction: There is a need of developing alternative compounds to overcome the multidrug resistance in ESBL producers. Biosynthesized silver nanoparticles (AgNPs) play an important role as the new antimicrobial compounds. AgNPs can control the growth of different organisms by releasing silver ions.
Objectives: To provide a method for the biosynthesis and characterization of AgNPs from Pseudomonas aeruginosa ATCC 27853 and to study its antibacterial activity against ESBL producing gram negative bacilli.
Methods: AgNPs were synthesized from Pseudomonas aeruginosa ATCC 27853. Characterization was done by UV spectroscopy, FTIR and SEM. Agar well diffusion method was employed to study the antibacterial activity of AgNPs and MIC was calculated by standard broth micro- dilution method.
Results: 575 ESBL producers have been isolated from different clinical specimens. Biosynthesis of AgNPs was done under controlled physicochemical parameters. In UV spectrophotometer, the maximum absorption of UV rays was seen at 422 nm. FTIR identified the role of biological molecules in the reduction of silver nitrate to silver nanoparticles. In SEM analysis, spherical shaped AgNPs showed the mean size of 26.187 ± 9.109 nm. In agar well diffusion method, antibacterial activity of AgNPs was seen at 10 and 20 μg/ml of AgNPs and MIC was in a range of 2.30 μg/ml - 3.28 μg/ml of AgNPs.
Conclusions: Green synthesis of AgNPs provides easiest method to modify the important properties of silver in the form of silver nanoparticles having a good antibacterial activity which have promising applications in various branches of medicine.