Investigation of the Phytochemical Composition and Cytotoxic Properties of Antidesma menasu (Tul.) Mull.Arg. Extract in Human Pancreatic Cancer Cells

Abstract

Antidesma menasu (Tul.) Mull.Arg. belongs to the family Phyllanthaceae. The plant is known for its anti-inflammatory and analgesic properties. Traditionally, the plant is used for the treatment and management of pain associated with arthritis and lower back pain. It is also used for the treatment of inflammatory swellings. In the present study, phytochemical analysis and assessment of cytotoxic activity of the ethanolic extract of A. menasu were carried out. The ethanolic extract was prepared by the cold extraction method and subjected to qualitative phytochemical analysis. The phytochemical investigations confirmed the presence of flavonoids, alkaloids, glycosides, steroids and terpenoids. The cytotoxic activity of the extract was evaluated by MTT assay against the human MiaPaCa-2 cell line. The ethanolic extract of A. menasu exhibited a significant cytotoxic activity against the human MiaPaCa-2 cell line with an IC50 value of 285.97 ± 1.78 μg/ml. Further studies are underway to explore the molecular mechanisms and identify the active phytochemical agents.