A Clinical Evaluation of the Incidence of Peri-Implantitis by Post-Surgical Evaluation of Implant Healing Marker/C-Reactive Protein Levels Using Latex Enhanced Nephelometry (LEN) in Implant Surgeries Coupled with Platelet-Rich Plasma and Ozone Interventio

Abstract

Aim: This study aims to evaluate the incidence of peri-implantitis by post-surgical evaluation of implant healing marker (C-reactive protein) levels using latex-enhanced nephelometry (Len) in implant surgeries coupled with platelet-rich plasma and ozone interventions.

Materials and Methods: This study involved 50 patients with missing right mandibular posterior teeth seeking prosthetic replacement, who 30 preferred implant-supported prostheses. Eligible participants were aged 21-35 with adequate bone quality, while smokers, those with mental instability, pregnant individuals, and those with poor bone quality were excluded. Informed consent was obtained, and 6 ml of blood was collected for platelet-rich plasma (PRP) extraction. Ozone was generated and infused into water. Before implant placement, patients received a chlorhexidine rinse and local anesthesia. They were divided into two groups: Group 1 (n=15) received PRP therapy and Group 2 (n=15) received ozone therapy. C-reactive protein (CRP) levels were measured within 24 and 48 hours after implantation.

Statistical Analysis and Results: This study examined 30 patients aged 21 to 35 with missing mandibular posterior teeth. Platelet-rich plasma (PRP) was collected preoperatively, and patients were divided into two groups: Group 1 (15 patients) received PRP-enhanced implants, while Group 2 (15 patients) underwent ozone therapy. C-reactive protein (CRP) levels were measured within 24- and 48-hours post-surgery. Group 1 had CRP levels of 2.01 ± 0.14 mg/dl at 24 hours and 1.20 ± 0.12 mg/dl at 48 hours after implant placement. Group 2 showed lower levels of 1.05 ± 0.22 mg/dl at 24 hours, decreasing to 0.59 ± 0.09 mg/dl at 48 hours after implant placement, indicating ozone therapy's effectiveness in reducing inflammation. Statistical analysis was performed using one-way ANOVA.

Conclusion: This study concluded that there is an increased level of CRP within 24 hours post-surgery for both groups, with a decline after 48 hours. Notably, the ozone therapy group had lower CRP levels than the PRP group at both time points; suggesting ozone therapy may reduce CRP levels more effectively. Future long-term studies are recommended to validate these findings for clinical practice.