Analytical Method Development and Validation Analysis for Quantitative Assessment of Triflumezopyrim by HPLC procedure

Abstract

The precise, systematic, explicit, particular, linear, exact and robust scientific method was developed and validated for the assay of Triflumezopyrim  in SBT TRIFLUMEZOPYRIM 10 SC fungicide. Presently utilized Triflumezopyrim as a working standard having limit for assay of Triflumezopyrim in SBT TRIFLUMEZOPYRIM 10 SC (CILPYROX) fungicide  are not less than 95.0%. Acetonitrile, water in the ratio (70:30 v/v) used as mobile phase and flow rate 1.0 ml / min. with 15 minutes run time. The detection was carried at 265 nm with column c18 - 250mm x 4.6mm x 5µ and ambient column temperature was maintained. The linearity of this method was found to be linear with a coefficient of regression at 0.999  in the concentration range of 50% to 160%. The linear regression equation was y=2846.0 x+89.55. The present developed HPLC method is detected to be suitable. The analytical solution was detected to be stable up to 48 Hrs at room temperature.