Extraction of Keratin from Human Hair with the Production of Biofertilizer

Abstract

Keratin has been attracting interest due to its stability against enzymatic degradation thereby allowing more predictable degradation profile for tissue regeneration. While the efficacy of keratin has been demonstrated indifferent models, there has been no systematic study to investigate and compare the different routes of keratin extraction from human hair. Here, undertaken work was designed to optimize the parameters for the keratin extraction from human hair with production of biofertilizer. Keratin extraction from human hair, hair dissolved in NaOH and stirred at 400C. The aliquot was then centrifuged at pH of 2-3.5, following which the precipitated keratin was extracted, dried and pulverized. The conformation study of the extracted keratin was done by performing xanthoproteic test, quantification of protein by Lowry’s method and separation of protein by SDS-PAGE. The keratin powder was compared with infected leaf by diffusion method. To prepare the foliar spray. However, to study the fertilizing property foliar spray from keratin, they are applied on seeds and recovered its biometric characteristics for 60 days. These results provide new insight into the extraction of keratin from human hair with implications for its use as an organic fertilizer.