Development and Validation of UV Spectrophotometric and HPLC methods for the Concurrent determination of Tramadol and Etoricoxib

Abstract

This study's goal is to outline the creation of an analytical technique for concurrently estimating two medications, Tramadol HCL and Etoricoxib, in a combination formulation shows better analgesic effect than individual drug. As such, there is no analytical method available for Qualitative and Quantitative determination of particular drugs in both combinations. Different analytical techniques can be applied for multicomponent analysis including: spectrophotometer, chromatography and electrophoresis. The proposed approach, the analytical methods of qualitative and quantitative analysis for combination of Tramadol and Etoricoxib have been using employing a UV spectrophotometer and RP-HPLC. Absorption The subtraction technique employed in simultaneous determination utilising a UV Spectrophotometer. In high performance liquid chromatography, separation carried out using a Phenomenex C18 column with a mobile phase of acetonitrile (65:35 v/v) and phosphate buffer (20 mM) at a flow rate of 1.02 ml/min. The parameters validated in accordance with ICH requirements, and it discovered that they had acceptable levels of accuracy, precision, repeatability, and robustness. Because of this, the procedure is quick and exact, and it may be used to identify both medications at the same. The objective of validation of analytical procedures is to demonstrate that it is suitable for its intended purpose.