Synthesis and Cytotoxic Activity of Novel Vit E infused Nanogel Formulation: An In Vitro Study

Abstract

Background: Vitamin E, a group of fat-soluble compounds first discovered in 1922, is valued for its various properties and includes tocopherols and tocotrienols. This study introduces a novel vitamin E-infused nanogel formulated with biodegradable chitosan nanoparticles, which enhances targeted drug delivery. This present study aims to synthesize a novel vitamin E-infused nano gel formulation and to evaluate the cytotoxic effects on the oral cancer cell line.

Materials and Methods: Formulation was prepared by combining 5 grams of Vitamin E powder, 0.5 grams of Chitosan, 0.2 milliliters of Tween 80, and 10 milliliters of Monosodium Phosphate in 90 milliliters of distilled water. This mixture was then heated to 55°C for 30 minutes while stirring with a magnetic stirrer. After heating, the mixture was lyophilized to form a gel. The cytotoxicity of the formulation was assessed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on the oral cancer cell line.

Results: The cytotoxic activity of the vitamin E-infused nanogel was evaluated at various concentrations, including 20, 40, 60, 80, 100, 120, 140, 160, 180, and 200 µg/mL, and was compared to both a control and a negative control group. The results showed a dose-dependent decrease in cell viability, indicating significant cytotoxic effects of the formulation on oral cancer cell lines. The formulation has shown high efficacy at a concentration of 120 µg/mL

Conclusion: This study highlights the potential of a vitamin E-infused nanogel with enhanced cytotoxic activity against oral cancer cell lines. Utilizing biodegradable chitosan nanoparticles, the nanogel ensures targeted drug delivery, improves adherence, and reduces side effects. It shows promise as an effective alternative for treating oral potentially malignant disorders and mucocutaneous conditions. Future research should explore its efficacy through in-vivo studies and clinical trials.