Invitro Antimicrobial Effect of Some Major Plants on Streptomyces Gelaticus and Identification of Potent Bioactive Chemical Metabolites using GC-MS Analysis

Abstract

The aim of this study was to identify the compounds synthesized by Streptomyces gelaticus and to determine the antibacterial, antifungal and in vitro plant extract antimicrobial activity. Methanol extract of Streptomyces gelaticus was examined for presence of bioactives using GC-MS data and, it was then subjected to antibacterial and antifungal assay in vitro.

The degree of bioactivity of methanolic extract of Streptomyces gelaticus and standard antibiotics against six different fungal and yeast species was tested Alternaria alternate (7.03 ± 0.04 and 9.01 ± 0.04), Aspergillusflavus (8.09 ± 0.02 and 4.06 ± 0.06), Candida albicans (0.97±0.01 and 9.03±0.07), Cladosporiumherbarum (6.10 ± 0.02 and 7.05 ± 0.06), Fusariumoxyporum (5.04 ± 0.06 and 0.83 ± 0.05),In the current investigation, bioactivity of the methanolic extract of Streptomyces gelaticus and the conventional antibiotics Rifambin and Cefotoxime against the five different infections that were examined was investigated E. coli (4.01±0.03, 3.35±0.03 and 2.61±0.01), Staph. aureus (6.11±0.04, 2.48±0.01 and 2.67±0.01), P. mirabilis (5.46±0.03, 2.70±0.01 and 1.09±0.01), Staph. Epidermidis (5.81±0.04 ,2.00±0.01 and 3.06±0.02).