Green Synthesis of Camellia Sinensis-Mediated Silver Nanoparticles Incorporated Hyaluronic Acid, Poly Vinyl Alcohol and its Antimicrobial Activity Against Oral Pathogens and its Biocompatibility Through Embryonic Toxicology Evaluation, Cytotoxic Effect an

Abstract

The study investigates the synergistic potential of a novel nanocomposite combining green tea extract, silver nanoparticles (AgNPs), and hyaluronic acid (HA) for its antioxidant, anti-inflammatory, and antimicrobial properties. Green tea, rich in polyphenols, offers potent antioxidant and anti-inflammatory effects, while silver nanoparticles are renowned for their broad-spectrum antimicrobial activity. Hyaluronic acid, a biocompatible polymer, serves as a stabilizing and bioactive agent, enhancing the biocompatibility and efficacy of the nanocomposite. The synthesis of the green tea-AgNP-HA nanocomposite was optimized using a green synthesis approach, ensuring eco-friendliness and minimizing toxicity. Comprehensive in vitro assays demonstrated significant antioxidant activity, as indicated by DPPH radical scavenging, along with notable anti-inflammatory effects in LPS-stimulated macrophage models. Additionally, the nanocomposite exhibited robust antimicrobial efficacy against common oral pathogens, including Streptococcus mutans and Candida albicans. These findings suggest that the green tea-AgNP-HA nanocomposite holds promise as a multifunctional therapeutic agent in the management of oral diseases, with potential applications in preventing and treating infections, reducing inflammation, and promoting tissue regeneration. Further in vivo studies are recommended to explore its clinical efficacy and safety.