Evaluation of Anti-anxiety Activity of Ethanolic Leaves Extract of Terminalia Arjuna in Wistar Albino Rats
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Abstract
Present study evaluated the anti-anxiety activity of leave extract of Terminalia Arjuna in experimental rats. Terminalia Arjuna is the well known herbal plant used for treatment of various diseases such as hypertension, heart disease, chest pain, asthma, wound healing and also CNS disorders. Arjuna plant is the richest source of Alkaloids, glycosides, flavonoids and tannins which has major role in treatment of various diseases. Present study is carried out by ethanolic extraction of Arjuna leaves by soxhlation method. Preliminary phytochemical analysis of leaves extract was carried out to confirm the presence of such metabolites. All the animals were divided into five groups; normal control, disease control, standard, test-1 and test-2 groups. Diazepam 2mg/kg was used as standard treatment drug. Test 1 group received Arjuna leave extract 200mg/kg oral and test group 2 was treated with Arjuna leaves extract 400mg/kg. Anti-anxiety activity in experimental animals had been evaluated by Elevated plus maze test and Light/dark exploration method. The result obtained from experiments was concluded that, the ethanolic leaf extract of Terminalia Arjuna having significant activity by EPM test (188±9.1) for open arms and Light/dark exploration test (239.68±9.2) for light box as compared to other animal groups (P<0.05). Histopathological findings showed the normal morphological structures of hippocampal sections of normal control animal group and test 2 animal groups. The result obtained from study was concluded that, the Arjuna leaves extract having significant anti-anxiety activity.