Isolation and Characterization of Indigenous Bacterial Strains from Constructed Wetland with Biofertilizer Potential

Abstract

Recent advancements in agricultural practices have significantly increased the use of chemical fertilizers, leading to soil fertility decline and heightened chemical toxicity. This study investigates the isolation of phosphate-solubilizing bacteria (PSB) and nitrogen-fixing bacteria (NFB) from rhizosphere soil, with the aim of producing effective biofertilizers. Conducted at constructed wetland the research identified seven distinct bacterial isolates capable of enhancing soil NPK values. These isolates demonstrated resilience across varying environmental conditions, including temperature, salinity, and pH levels. Results from constructed wetland studies and in vitro assays indicated that these bacteria effectively improve soil health and nutrient availability, showcasing their potential as sustainable alternatives to chemical fertilizers. The implications of this research extend to restoring nutrient cycles and enhancing plant growth in diverse agricultural settings, particularly in regions facing soil degradation. Future applications of these biofertilizers may support sustainable farming practices while promoting entrepreneurial opportunities in agribusiness.