Design, Optimization and Characterization of Achyranthes Aspera Extract Loaded Nanogel for Effective Treatment of Acne and Aging
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Abstract
The Achyranthes aspera extract loaded nanogel was successfully prepared optimized and in-vitro and in-vivo evaluated to enhance the potential of herbal dug and stabilized the formulation. The crude extract was screened for the presence or absence of secondary metabolites. Physiochemical analysis of dry powders of Achyranthes aspera leaves was performed. Hyaluronic acid and polyvinyl alcohol were water soluble substances but dissolved slowly so the warm water was used to solubilize the polymers. Various percentages of polymers were mixed. Free radical scavenging activity of samples was measured using DPPH and The ABTS radical scavenging methods. The results exposed that the particle size was 302 ± 12 nm, Gelling time 6.3 ± 0.26 min and entrapment efficiency found (89.92 ± 0.92) % for AA-4 batch. The AA-4 was selected as optimized batch for the further evaluation. The investigation results of antioxidant activity of Achyranthes aspera extracts and extract loaded nanogels were performed as DPPH and ABTS radical scavenging activity. It was concluded that the Achyranthes aspera extracts and nanogel AA-4 possessed potential to scavenge free radicals it will helps to reduces aging because the main cause of aging is free radicals. It was also concluded that the developed nanogel provides unique mechanism to deliver drug effectively at inflamed ear of mice when applied topically. Both delivery and biological activity are significantly enhanced, which was found not to be pro-inflammatory. In a therapeutic context, this Achyranthes aspera loaded nanogel and A. aspera nanogel delivery system would potentially useful for the topical delivery of a drugs.