Osmium as Reagent for Quantitative Analysis of Anti-Cancer Drug Mesna as Trithiocarbonate Derivative

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Teena Sainia, Sunita Bishnoia, Rupali Shrivastavaa

Abstract

A sensitive and rapid spectrophotometric method was used to determine Mesna trithiocarbonate, using osmium chloride. Effect of temperature and pH on the complexation of osmium (II) with sodium2-mercaptoethanesulphonate trithiocarbonate was studied. A plot between absorbance and mole ratio of reagent shows that metal has been consumed completely at mole ratio one and further addition of ligand produces no more complexation. A good linear relationship was observed over the range 2.78x10-1 g/ml to 5.57 g/ml of mesna trithiocarbonate in calibration curve. The effective molar absorptivity was calculated from the slope of the calibration graph to be found 1.6x102 ml mol-1 cm-1. The relative standard deviation (RSD%) was found to be 1.16 %. The correlation coefficient value close to +1 indicates a strong direct relationship between two variables. The relationship between absorbance and concentration is very close and direct. Hence, the developed method can be utilized successfully to determine the concentration of sodium 2-mercaptoethanesulphonate trithiocarbonate and drug mesna in pharmaceutical samples by simply knowing its absorbance.

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