From Attachment to Dispersion: Understanding Protein-Mediated Dynamics in Staphylococcus Aureus Biofilms Via Protein Pathways in Staphylococcus Aureus Biofilm Dispersal: Insights from Lab-Scale Experimental Models

Abstract

Introduction: By emphasizing the seriousness of medical device associated infections and how Staphylococcus aureus biofilms exacerbate the situation of Catheter associated urinary tract infection (CAUTI) and implant associated osteomyelitis. It highlights the necessity of investigating the features of silicone catheter biofilm formation & implants in order to understand the underlying mechanisms and create practical prevention measures in a lab scale model [1].

Objective: The study aims to investigate in detail the growth and development patterns in Staphylococcus aureus biofilms, particularly those found inside silicone catheters and implants. To investigate the specific protein pathways involved in the dispersal of Staphylococcus aureus biofilms using controlled lab-scale experimental models, with the aim of gaining insights into the mechanisms underlying biofilm dispersion and potential targets for intervention strategies

Materials and methods: Biofilm development was observed in silicone urine catheter tubes and stainless steel & titanium orthopedic implants for a particular period. In order to determine if biofilms are developed, it is evaluated using the modified and Congo red methods, scanning electron microscopy (SEM) analysis and antibiotic susceptibility assay to evaluate biofilms against certain antibiotics. Crude protein isolation from the S aureus biofilm solution was studied.

Results: The research findings demonstrated the development and existence of Staphylococcus aureus biofilms within the walls & surface of silicone urinary catheter & implants. The isolation of the biofilm protein provided evidence of the formation of S aureus biofilm in the lab scale module.