Simultaneous Estimation of Dapagliflozin and Teneligliptin by Analytical Method Development Using HPLC

Abstract

Analytical methods need to be validated or revalidated prior to their introduction into routine analyses. Chromatography is an analytical techniques based on the separation of molecules due to differences in their structure and/or composition. In general, Chromatography involves moving a sample through the system over a stationary phase. The molecules in the samples will have different affinities and interaction with the stationary support, leading to separation of Molecules. Samples components that display stronger interaction with the stationary phase will move more slowly through the column than components with weaker interaction. Different compounds can be separated from each other as they move through the column. Chromatographic separation can be carried out using a variety of stationary phases. High-Performance liquid chromatography (HPLC) is types of liquid Chromatography used to separate and quantify compounds that have been dissolved in Solution.