A Comparison Study Between Manual Semen Analysis with that of Computer Assisted Semen Analysis.

Abstract

Objectives: The study aimed to check the quality of the computer-assisted semen analysis (CASA) system compared to the reference manual method and standardisation of the computer-assisted semen assessment.

Material and methods: The study was conducted between October 2023 and March 2024 at MMCRI, Mysore. The samples underwent manual and computer-assisted concentration, motility, and morphology assessment. A total of 68 samples were examined twice: manually, according to the 2021 WHO recommendations, and with CASA, using the program settings provided by the manufacturer. The p-value of p < 0.05 was considered statistically significant.

 Results: Statistically significant differences were found among all investigated sperm parameters, except for non-progressive motility, as measured with CASA and manually. In the group of patients where all analyses with each method were performed twice on the same sample, we found no significant differences between both assessments of the same probe, neither in the samples analysed manually nor with CASA. However, the standard deviation was higher in the CASA group.

 Conclusions: Our results suggest that computer-assisted semen analysis requires further improvement for a broader application in clinical practice.