Development and Validation of UV Visible Spectrophotometric Method for Estimation of Quercetin

Abstract

Introduction: Quercetin is a naturally occurring flavonoid chemical that is found in many different plant species and has a range of pharmacological properties. Among flavonoids, quercetin is the most common, having three rings and five hydroxyl groups.

Objectives: The objective of this work is to validate a suggested methodology for quercetin in its purified form and to provide a simple UV visible spectrophotometric technique for measurement. Additionally, the investigation aims to utilize an UV visible twin beam spectrophotometer to measure pharmaceutical formulations at maximum absorption 370 nm.

Methods: The study focused on generating a specific, linear, accurate and precise UV spectrophotometric method for estimation of quercetin. Statistical validation followed International Conference of Harmonization (ICH) specifications. The method's parameters were assessed within a concentration range of 0.2-1 µg/ml, with recovery rates specifying accuracy and low % relative standard deviation (RSD) values confirming precision. Limits of detection (LOD) and quantification (LOQ) for quercetin were determined.

Results: A coefficient of correlation of 0.9995 was discovered in accordance with Beer's law. After determining the technique's sensitivity, the limit of detection and limit of quantification were determined to be 0.043µg/ml and 1.303µg/ml, respectively.

Conclusions: The developed UV spectrophotometric method proved suitable for the quantitative estimation of quercetin, offering rapid and accurate analysis. The results underscore the method's linearity, accuracy, robustness, LOD, LOQ, area under the curve and precision within the specified concentration range.