Development and Validation of NP-HPTLC Method for Estimation of Emtricitabine in Bulk and Pharmaceutical Dosage Form

Abstract

Objective of the present work is to develop and validate a simple, precise, selective, cost effective, and sensitive, high-performance thin layer chromatography (HPTLC) method for the estimation of emtricitabine in bulk and pharmaceutical dosage form. The optimized mobile phase contained of toluene: methanol 3.5:1.5(v/v). The method used HPTLC precoated silica gel 60 F254 as a stationary phase. Densitometric estimation of emtricitabine was carried out in the absorbance mode at 240nm. The method was linear in the range of 300-1800 ng/band. The correlation coefficient is 0.998 and slope 3.611, intercept 1696. The limit of detection and limit of quantitation were 10.93 and 33.14 respectively. The developed method is precise accurate, sensitive for analysis of emtricitabine in bulk and pharmaceutical dosage form.