Development and Characterization of Thujone Loaded Pegylated Nanoparticle Delivery for Enhanced Brain Targeting

Abstract

The goal of this study was to create biodegradable pegylated chitosan nanoparticles and assess how well they might target specific areas of the brain on a single platform. The PEGylated chitosan nanoconjugate was created and tested to transfer Thujone, a natural anti-Alzheimer constituent to the brain. Using the ionic gelation process, the nanoconjugate (TPNs) was created and assessed for a number of optical and in-vitro parameters. The Thujone loaded PEGylated nanosystem had IC50 values of 0.42, 0.49, 0.67, and 0.75 µM, according to the MTT experiment conducted on UCSD229i-SAD1-1 human astrocytoma cells. Enhanced uptake and biodistribution tests by confocal microscopy and apoptosis assay, which notably revealed increased accumulation of nanoconjugate in brain as compared to free Thujone solution, are authenticated by the in vitro cell lines evaluation. When compared to free Thujone solution, the targeting potential of TPNs was shown to be twice as significant. Conclusion: In order to improve the delivery of anti-Alzheimer medications to the brain for better treatment outcomes, bidoegradablePEgylated chitosan nanoconjugate may be employed as a possible nano-targeting to add PEGylated chitosan nanoparticles.