Isolation, Characterization and Optimization of Saccharomyces Cerevisiae Jssatpvr1 for Protease Production

Abstract

Soil is a rich reservoir of diverse microorganisms with immense biotechnological potential, including those capable of producing proteases, enzymes crucial for various industrial processes. This study aimed to isolate soil microorganisms and identify protease-producing strains for potential biotechnological applications. Soil samples were collected from diverse geographical locations, and serial dilution techniques were employed to isolate microbial colonies. The isolates were screened for protease production using skim milk agar plates supplemented with casein as a substrate. Positive isolates were further characterized biochemically and molecularly.

Results revealed a diverse microbial community in the soil samples. Among these, several strains exhibited significant protease activity, indicating their potential for industrial applications. Biochemical characterization revealed the proteases to be of various types, including serine, cysteine, and metalloproteases, suggesting diverse enzymatic capabilities within the isolated strains. Molecular identification using 16S rRNA sequencing and ITS region analysis identified the predominant protease producers as saccharomyces cerevisiae.

Furthermore, the protease-producing strains demonstrated robust enzymatic activity under a wide range of environmental conditions, including pH and temperature variations, highlighting their potential for industrial processes requiring enzyme stability under diverse conditions. Moreover, the production of proteases by indigenous soil microorganisms suggests their adaptation to local environmental conditions, making them promising candidates for biotechnological applications in waste management, bioremediation, and pharmaceutical industries.

In conclusion, this study underscores the importance of soil as a reservoir of diverse microorganisms with biotechnological significance. The isolated protease-producing strains exhibit promising enzymatic capabilities and environmental adaptability, laying the foundation for further exploration and utilization of soil microbial diversity for sustainable industrial processes